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Among seven selected alpha-hydroxy acids, tartaric acid exhibited potential for improving the chromatographic separation of cyanidin-3--glucoside was the main anthocyanin in haskap berry, mulberry, and blackberry, and the three berries were selected for further method development and validation.
The optimized mobile phase was a mixture of ethanol and a 0.25 mol L-glucoside was less than 1.8 min, and an optimum separation of anthocyanins was achieved in 4 min.
The developed method was validated in terms of linearity, limits of detection and quantification, precision, repeatability, stability, and recovery.
The calibration curve revealed good linearity (-glucoside from the berry samples were between 99% and 101%, a satisfactory level.
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